Chinese hamster ovary cells lacking GM1 and GD1a synthesize gangliosides upon transfection with human GM2 synthase.
Biochim Biophys Acta
; 1354(2): 153-8, 1997 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-9396632
ABSTRACT
GM3-positive Chinese hamster ovary cells (CHO-K1 cells) lack the ability to synthesize GM2 and the complex gangliosides GM1 and GD1a from [3H]Gal added to the culture medium. However, they acquire the ability to synthesize GM2 and to synthesize and immunoexpress complex gangliosides upon transient transfection with a cDNA encoding the human GM3N-acetylgalactosaminyl transferase (GM2 synthase). The activities of endogenous GM1- and GD1a-synthases in the parental cell line and in cells transfected with the plasmid with or without the GM2 synthase cDNA were essentially identical and comparable in terms of specific activity with the endogenous GM3 synthase. Results indicate that glycosyltransferases acting on GM2 to produce GM1 and GD1a are constitutively present in CHO-K1 cells, and that the expression of their activities depend on the supply of the acceptor GM2. In addition, these results lend support to the notion that GM2 synthase is a key regulatory enzyme influencing the balance between simple and complex gangliosides.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Transfección
/
N-Acetilgalactosaminiltransferasas
/
Gangliósidos
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1997
Tipo del documento:
Article
País de afiliación:
Argentina