Characterization of the 5' flanking region of the human NPT-1 Na+/phosphate cotransporter gene.
Biochim Biophys Acta
; 1396(3): 267-72, 1998 Mar 13.
Article
en En
| MEDLINE
| ID: mdl-9545579
ABSTRACT
To elucidate the expression and regulation of the human type I Na+/phosphate transporter gene (NPT-1), the 5' flanking region of the NPT-1 gene was cloned, and its nucleotide sequence and function were determined. A genomic clone that contained approximately 14.0 kb of the 5'-flanking region of the NPT-1 gene was isolated. A single transcription start site was located 104 base pairs (bp) upstream of the 3' end of exon 1. In addition to the sequence of the 5'-flanking region contained a sequence weakly homologous to a TATA box at position -41 to -36 and many transcriptional regulatory elements. Transient expression revealed that a 45-bp region of proximal to exon 1, which contained TATA-like sequence, was sufficient for promoting luciferase expression in OK-cells derived from opossum kidney proximal tubule.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Portadoras
/
Regiones Promotoras Genéticas
/
Simportadores
Límite:
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1998
Tipo del documento:
Article
País de afiliación:
Japón