Development and evaluation of polymerase chain reaction tests as an aid to diagnosis of swine dysentery and intestinal spirochaetosis.
Lett Appl Microbiol
; 26(2): 126-30, 1998 Feb.
Article
en En
| MEDLINE
| ID: mdl-9569695
ABSTRACT
Polymerase chain reaction (PCR) tests were established for detection of Serpulina hyodysenteriae, the agent of swine dysentery, and S. pilosicoli, the agent of intestinal spirochaetosis. Both reactions were specific when tested with DNA from 107 strains of various intestinal spirochaetes. For diagnostic use, faeces were plated to selective medium, and diatomaceous earth extraction used to obtain DNA prior to PCR. This procedure detected 10(3)-10(4) cells of either organism seeded into 0.2 g of faeces. When applied to 63 samples from pigs of eight piggeries naturally infected with either S. hyodysenteriae or S. pilosicoli, both PCRs were specific, more rapid, and detected more positive samples than did routine faecal culture and isolation.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Infecciones por Spirochaetales
/
Enfermedades de los Porcinos
/
Reacción en Cadena de la Polimerasa
/
Brachyspira hyodysenteriae
/
Disentería
/
Enfermedades Intestinales
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Lett Appl Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Australia