Transgenic Cre recombinase expression in germ cells and early embryogenesis directs homogeneous and ubiquitous deletion of loxP-flanked gene segments.
FEBS Lett
; 438(1-2): 76-80, 1998 Oct 30.
Article
en En
| MEDLINE
| ID: mdl-9821962
ABSTRACT
We report on the establishment of a transgenic mouse line expressing Cre recombinase under control of the c-kit promoter. Expression of Cre recombinase was only observed in late spermatogenesis and oogenesis, however, Cre-mediated deletion of floxed gene segments occurred at this stage as well as in early embryogenesis. As a consequence of this, a chimeric distribution of loxed alleles was found in a large fraction of these mice. The chimerism was very homogeneous in different organs and tissues of the same individual but varied between different individual offspring. The potential uses for this mouse line are discussed.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Virales
/
Regiones Promotoras Genéticas
/
Eliminación de Secuencia
/
Regulación del Desarrollo de la Expresión Génica
/
Integrasas
/
Embrión de Mamíferos
/
Células Germinativas
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1998
Tipo del documento:
Article
País de afiliación:
Suecia