Mycobacterium paratuberculosis and Mycobacterium avium subsp. silvaticum DNA cannot be detected by PCR in Crohn's disease tissue.
Gastroenterol Clin Biol
; 22(8-9): 675-8, 1998.
Article
en En
| MEDLINE
| ID: mdl-9823555
ABSTRACT
BACKGROUND:
The etiology of Crohn's disease remains unknown. A putative mycobacterial cause of the disease is still controversial.AIMS:
To assess the mycobacterial hypothesis in Crohn's disease using a polymerase chain reaction technique. PATIENTS ANDMETHODS:
Nested polymerase chain reaction with primers on the 16S-rRNA coding region (16S-rDNA) and with primers specific both to the insertion sequences (IS) 900, and IS 901/902 were used to amplify Mycobacterium paratuberculosis or Mycobacterium avium subsp. silvaticum DNA in frozen endoscopic intestinal biopsies or surgical resection specimens from patients with Crohn's disease (n = 47 25 endoscopic biopsies and 22 surgical resection samples, +/- lymph nodes), ulcerative colitis (n = 27), and non inflammatory bowel diseases (n = 20 colonic tumors and diverticulitis). Positive as well as negative controls were used throughout the study.RESULTS:
All strains of Mycobacterium paratuberculosis and Mycobacterium avium subsp. silvaticum tested were positive for both primer systems. Of the 94 biopsies tested, 5 (2 Crohn's disease, 1 ulcerative colitis and 2 controls) were positive with the 16S-rDNA primers but did not correspond to Mycobacterium paratuberculosis or Mycobacterium avium subsp. silvaticum. None of the specimens was positive with the IS primers.CONCLUSION:
These results do not support the hypothesis that Mycobacterium paratuberculosis, or Mycobacterium avium subsp. silvaticum play a role in Crohn's disease.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN Bacteriano
/
Enfermedad de Crohn
/
Mycobacterium avium subsp. paratuberculosis
/
Mycobacterium avium
Límite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Gastroenterol Clin Biol
Año:
1998
Tipo del documento:
Article