Resistance to apoptosis of harringtonine-resistant HL60 cells induced by tetrandrine.
Zhongguo Yao Li Xue Bao
; 17(6): 545-9, 1996 Nov.
Article
en En
| MEDLINE
| ID: mdl-9863153
ABSTRACT
AIM:
To study the mechanism of resistance to apoptosis in the harringtonine (Har)-resistant HL60 cells with tetrandrine (Tet).METHODS:
Growth inhibition, flow cytometry, DNA agarose gel electrophoresis, protein phosphorylation, and RNA dot hybridization.RESULTS:
The resistant cells had no cross resistance to Tet. Tet induced the sensitive but not the Har-resistant HL60 cells to apoptosis. The high phosphorylation of protein < 30 kDa occurred when the resistant cells were treated with Tet. Tet and Har increased the expression of c-myc mRNA in the sensitive HL60 cells. The expression of c-myc mRNA in the resistant cells was obviously decreased and almost not changed in treatment with Tet and Har.CONCLUSION:
Tet induced the sensitive but not the Har-resistant HL60 cells to apoptosis, and the resistance to apoptosis induced by Tet was associated with the high protein phosphorylation and reduction of the expression of c-myc mRNA.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Apoptosis
/
Bencilisoquinolinas
/
Alcaloides
/
Harringtoninas
/
Antineoplásicos Fitogénicos
Límite:
Humans
Idioma:
En
Revista:
Zhongguo Yao Li Xue Bao
Año:
1996
Tipo del documento:
Article
País de afiliación:
China