Role of Pasteurella multocida porin on cytokine expression and release by murine splenocytes.
Vet Immunol Immunopathol
; 66(3-4): 391-404, 1998 Dec 11.
Article
en En
| MEDLINE
| ID: mdl-9880114
ABSTRACT
The aim of this study was to verify whether Pasteurella multocida porin can affect the expression and release of IL-1alpha, IL-6, TNF-alpha, IL-4, IFN-gamma, IL-10 and IL-12 by murine splenocytes in vitro. P. multocida porin and lipopolysaccharide (LPS) were able to induce the release of IL-1alpha, IL-6, TNF-alpha, IFN-gamma and IL-12 in a dose-dependent fashion. The greatest release of these cytokines was obtained using P. multocida porin at a concentration of 5 microg ml(-1) and LPS at a concentration of 1 microg ml(-1). The time-courses of release showed that P. multocida LPS was able to stimulate the production of IL-1alpha, IL-6, TNF-alpha, IFN-gamma and IL-12 earlier than porin and at a greater rate. No effect was observed on IL-4 and IL-10 release under the same experimental conditions. P. multocida porin and LPS were also able to up-regulate the mRNA expression of IL-1alpha, IL-6, TNF-alpha, IFN-gamma and IL-12 p40. Our findings suggest that P. multocida porin is able to modulate inflammatory and immunological responses by affecting the release of several cytokines and the expression of their genes.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bazo
/
Citocinas
/
Pasteurella multocida
/
Porinas
Límite:
Animals
Idioma:
En
Revista:
Vet Immunol Immunopathol
Año:
1998
Tipo del documento:
Article
País de afiliación:
Italia