The use of dacron plates for DOT enzyme-linked immunosorbent assay (DOT-ELISA)
Mem. Inst. Oswaldo Cruz
; 86(4): 461-5, Oct.-Dec. 1991. tab
Article
em En
| LILACS
| ID: lil-109147
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BR1.1
RESUMO
Dacron (polyethylenetherephthalate) is proposed as a matrix for dot-ELISA procedures, as an alternative to nitrocellulose. Plates of dacron were partially hydrazinolyzed and hydrazide groups introduced were converted to azide groups. The derivative dacron-antigen was covalently linked on to the plates through these azide groups. The derivative dacron-antigen was exaustively washed according to CROOK and antigen was still fixed onto the plates. Protein F1A purified from Yersinia pestis was used as a model. Triration of sera from immunized and non immunized rabbits against this protein was carried out by employing the dot-ELISA method. No significant difference was observed using dacron-antigen and nitrocellulose-antigen preparations. Howwvwe, both procedures showed to have a significant better performance in comparasion with the passive hemagglutination method. The specificity and reproductibility of the dot-ELISA assay using both preparations showed a similar behaviour. Nitrocellulose preparation was stable at 4 graus Centígrados, 28 graus centígrados and -20 graus centígrados for 90 days, whereas the dacron-antigen derivative was stable only when stored at 4 graus centígrados. Dacron-antigen derivative could be re-used when the spot developing was proceeded using 4-chloro-1-naphtol as substrate
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Immunoblotting
/
Polietilenotereftalatos
/
Colódio
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
1991
Tipo de documento:
Article