An indirect immunofluorescence antibody test employing whole eggs as the antigen for the diagnosis of abdominal angiostrongyliasis
Mem. Inst. Oswaldo Cruz
; 106(4): 390-393, June 2011. tab
Article
em En
| LILACS
| ID: lil-592179
Biblioteca responsável:
BR1.1
ABSTRACT
Abdominal angiostrongyliasis is a potentially fatal zoonotic disease with a broad geographical distribution throughout Central and South America. This study assessed the performance of Angiostrongylus costaricensis eggs as the antigen in an indirect immunofluorescence assay for the determination of parasite-specific IgG and IgG1 antibodies. For prevalence studies, an IgG antibody titre > 16 was identified as the diagnostic threshold with the best performance, providing 93.7 percent sensitivity and 84.6 percent specificity. Cross reactivity was evaluated with 65 additional samples from patients with other known parasitic infections. Cross reactivity was observed only in samples from individuals infected with Strongyloides stercoralis. For clinical diagnosis, we recommend the determination of IgG only as a screening test. IgG1 determination may be used to increase the specificity of the results for patients with a positive screening test.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Imunoglobulina G
/
Anticorpos Anti-Helmínticos
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Infecções por Strongylida
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Angiostrongylus
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Antígenos de Helmintos
Tipo de estudo:
Diagnostic_studies
/
Risk_factors_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
/
Mem. Inst. Oswaldo Cruz
/
Mem. Inst. Oswaldo Cruz (Online)
/
Memórias do Instituto Oswaldo Cruz (Impresso)
/
Proceedings of the Instituto Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2011
Tipo de documento:
Article
/
Project document
País de afiliação:
Costa Rica