CD144, CD146 and VEGFR-2 properly identify circulating endothelial cell
Rev. bras. hematol. hemoter
; Rev. bras. hematol. hemoter;37(2): 98-102, Mar-Apr/2015. tab, graf
Article
em En
| LILACS
| ID: lil-746095
Biblioteca responsável:
BR408.1
ABSTRACT
Studies evaluating circulating endothelial cells by flow cytometry are faced by a lack of con- sensus about the best combination of monoclonal antibodies to be used. The rarity of these cells in peripheral blood, which represent 0.01% of mononuclear cells, drastically increases this challenge. Objective:
The aim of this study is to suggest some combinations of markers that would safely and properly identify these cells.Methods:
Flow cytometry analysis of circulating endothelial cells was performed applying three different panels composed of different combinations of the CD144, CD146, CD31, CD133, CD45 and anti-Vascular endothelial growth factor receptor-2 antibodies.Results:
In spite of the rarity of the events, they were detectable and presented similar interperson numbers of circulating endothelial cells.Conclusion:
The combination of markers successfully identified the circulating endothelial cells in healthy individuals, with the use of three different panels confirming the obtained data as reliable.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Células Endoteliais
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Rev. bras. hematol. hemoter
/
Rev. bras. hematol. hemoter. (Online)
/
Revista brasileira de hematologia e hemoterapia (Impresso)
Assunto da revista:
HEMATOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Brasil