The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Rúmen
/
Cabras
/
Lactobacillus plantarum
/
Duodeno
Limite:
Animals
Idioma:
En
Revista:
Braz. j. microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
/
Project document
País de afiliação:
China
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Rúmen
/
Cabras
/
Lactobacillus plantarum
/
Duodeno
Limite:
Animals
Idioma:
En
Revista:
Braz. j. microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
/
Project document
País de afiliação:
China
Consulta Detalhada
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