An antagonistic monoclonal antibody (B-N6) specific for the human neurotensin receptor-1.
Neuropeptides
; 32(3): 247-56, 1998 Jun.
Article
em En
| MEDLINE
| ID: mdl-10189059
ABSTRACT
The neuropeptide neurotensin (NT) interacts with two types of human receptors (hNTR) termed hNTR-1 and hNTR-2. This study describes a monoclonal antibody (MAb) specific for hNTR-1, B-N6. This MAb binds specifically to hNTR-1, but not to hNTR-2 transfected CHO cells. B-N6 and NT display a reciprocal competition and react in a similar way to trypsin, suggesting that the B-N6 epitope is at or close to the NT binding site on the third extracellular loop. Unlike B-N6, NT induces hNTR-1 internalization. Although neither NT-FITC nor B-N6 binding was detected by flow cytometry on different human cells, specific mRNA expression for hNTR-1 was detected in these cells. In CHO cells expressing hNTR-1 and a luciferase gene coupled to the krox24 reporter, B-N6 and the antagonist SR 48692 inhibited NT-induced intracellular activation of krox24 in a dose-dependent manner. From these results it is concluded that B-N6 is an antagonistic anti-hNTR-1 MAb.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Neurotensina
/
Proteínas Imediatamente Precoces
/
Anticorpos Monoclonais
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Neuropeptides
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
França