Channel-sizing experiments in multichannel bilayers.
Gen Physiol Biophys
; 17(4): 349-63, 1998 Dec.
Article
em En
| MEDLINE
| ID: mdl-10191420
ABSTRACT
The possibility of obtaining information about the radius of high and low conductance states of channels in multichannel membranes was tested experimentally. In spite of the interference of non-electrolytes on the numbers of channels that appeared in the membrane, the non-electrolyte-exclusion method was successfully adapted to multichannel bilayers to estimate the radius of the larger opening of the low conductance state of the channel induced by Staphylococcus aureus alpha-toxin. At the pH used, the channel transition to a low conductance state was accompanied by a decrease of the opening radius from 1.3 +/- 0.2 nm to 0.9 +/- 0.1 nm. The determination criteria for maximum size of a channel opening when using the non-electrolyte exclusion method is discussed.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Canais Iônicos
Idioma:
En
Revista:
Gen Physiol Biophys
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Uzbequistão