Co-transcription of Rhizobium meliloti lysyl-tRNA synthetase and glutamyl-tRNA synthetase genes.
FEBS Lett
; 449(1): 23-7, 1999 Apr 16.
Article
em En
| MEDLINE
| ID: mdl-10225420
ABSTRACT
An open reading frame encoding a putative polypeptide very similar to several lysyl-tRNA synthetases was found 10 nucleotides downstream of Rhizobium meliloti gltX encoding glutamyl-tRNA synthetase. Expression of this gene complemented a mutation in lysS of Escherichia coli and led to the overexpression of a polypeptide of the expected mass (62 kDa), thus confirming that it encodes R. meliloti lysyl-tRNA synthetase. Reverse transcription/polymerase chain reaction was used to demonstrate that this lysS gene is co-transcribed with gltX in R. meliloti. This is the first reported case of two immediately adjacent and co-transcribed genes encoding aminoacyl-tRNA synthetases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
Sinorhizobium meliloti
/
Glutamato-tRNA Ligase
/
Lisina-tRNA Ligase
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Canadá