OTX2 homeodomain protein binds a DNA element necessary for interphotoreceptor retinoid binding protein gene expression.
Mech Dev
; 82(1-2): 165-9, 1999 Apr.
Article
em En
| MEDLINE
| ID: mdl-10354480
ABSTRACT
Transcription of the human interphotoreceptor retinoid binding protein (IRBP) gene is strictly tissue specific, being restricted to retinal photoreceptors and pinealocytes. We have previously demonstrated that a sequence named A element, in the IRBP promoter is essential for IRBP gene transcription in vivo. Here we demonstrate that the human homeodomain protein OTX2 is present in nuclear extracts of IRBP expressing cells and specifically interacts with the IRBP A promoter element in vitro. OTX2, as well as CRX, a homeodomain protein very similar to OTX2, activates the human IRBP promoter in co-transfection experiments.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Fotorreceptoras
/
DNA
/
Proteínas de Ligação ao Retinol
/
Transativadores
/
Proteínas de Homeodomínio
/
Proteínas do Olho
/
Proteínas do Tecido Nervoso
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Mech Dev
Assunto da revista:
EMBRIOLOGIA
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Itália