Hypomethylation of the proximal and intronic regulatory regions of the IFN-gamma gene is not essential for its transcription by naive CD4+ T cells cultured with IL-4.
Immunol Lett
; 69(2): 239-45, 1999 Aug 03.
Article
em En
| MEDLINE
| ID: mdl-10482358
ABSTRACT
Recently, long-term preculture with IL-4 or IL-7 has been reported to induce IFN-gamma-producing ability in naive CD4+ T cells without stimulation via TCR. The mechanism of IFN-gamma-transcription in naive CD4+ T cells precultured with IL-4 was analyzed and compared with that in typical Th1 cells by focusing on the TATA proximal and first intronic regulatory regions of the IFN-gamma gene. Both regulatory regions in these IL-4-primed naive CD4+ T cells, which produce a large amount of IFN-gamma upon stimulation with PMA and ionomycin, were completely methylated in contrast to the same hypomethylated regions in Th1 cells. DNase I hypersensitive site analysis suggested that both regulatory regions in IL-4-primed naive CD4+ T cells were not active for IFN-gamma-expression. Moreover, we demonstrated that the composition of transcriptional factors that can bind to the proximal regulatory region is different between IL-4-primed naive CD4+ T cells and Th1 cells. These results indicated that the transcriptional machinery involved in the expression of the IFN-gamma gene by CD4+ T cells varied depending on their modes of differentiation in both the responsive regulatory regions and the specific nuclear factors.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Íntrons
/
Linfócitos T CD4-Positivos
/
Sequências Reguladoras de Ácido Nucleico
/
TATA Box
/
Interleucina-4
/
Interferon gama
/
Metilação de DNA
Limite:
Humans
Idioma:
En
Revista:
Immunol Lett
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Japão