Visualisation of plasmid replication intermediates containing reversed forks.
Nucleic Acids Res
; 28(2): 498-503, 2000 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-10606648
ABSTRACT
Blockage of replication forks can have deleterious consequences for the cell as it may prompt premature termination of DNA replication. Moreover, the blocked replication intermediate (RI) could be particularly sensitive to recombination processes. We analysed the different populations of RIs generated in vivo in the bacterial plasmid pPI21 after pausing of replication forks at the inversely oriented ColE1 origin. To achieve this goal, a new method was developed based on two-dimensional agarose gel electrophoresis. This method allows the isolation of specific RIs, even when they were rather scarce, from the total DNA. Here we describe the occurrence of RI restriction fragments containing reversed forks. These Holliday-like structures have been postulated but never observed before.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
DNA
/
Replicação do DNA
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Espanha