Human antibody response to longus type IV pilus and study of its prevalence among enterotoxigenic Escherichia coli in Bangladesh by using monoclonal antibodies.
J Infect Dis
; 181(6): 2071-4, 2000 Jun.
Article
em En
| MEDLINE
| ID: mdl-10837196
Mouse monoclonal antibodies (MAbs) were derived against longus (CS20), a type IV pilus expressed by human enterotoxigenic Escherichia coli (ETEC). One MAb (ICA39) detected longus in 56 (8.5%) of 662 ETEC isolates obtained from a routine surveillance of diarrheal stools from children and adults. Five patients with diarrhea from whom longus-positive ETEC were isolated were also recruited. Of these 61 isolates, 50 were positive for other colonization factors (CFs; 61% for CFA/II and 21% for CFA/I), and 11 were negative for any of the other 8 CFs that were tested. They were either positive for the heat-stable enterotoxin (ST; n=29) or for the heat-labile enterotoxin (LT) and ST (n=32). All longus-positive ETEC were confirmed by polymerase chain reaction to harbor lngA, the longus structural pilin gene. Sera and/or fecal extracts from the patients reacted with the 22-kDa pilin polypeptide in immunoblots and ELISA. These studies show that longus is prevalent among ETEC in Bangladesh and that longus gives rise to IgA antibody responses in patients.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fímbrias Bacterianas
/
Proteínas de Fímbrias
/
Escherichia coli
/
Anticorpos Antibacterianos
/
Anticorpos Monoclonais
Tipo de estudo:
Prevalence_studies
/
Risk_factors_studies
Limite:
Adult
/
Animals
/
Child, preschool
/
Female
/
Humans
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Bangladesh