Different regulation of phospholipase D activity in glioma C6 cells by sphingosine, propranolol, imipramine and phorbol ester.
Cell Signal
; 12(6): 399-404, 2000 Jun.
Article
em En
| MEDLINE
| ID: mdl-10889469
ABSTRACT
In has been found that sphingosine, propranolol, imipramine and phorbol ester (12-O-tetradecanoylphorbol-13-acetate, TPA) have a stimulatory effect on phospholipase D activity in glioma C6 cells. The cells were prelabelled with [1-(14)C]palmitic acid and phospholipase D-mediated synthesis of [(14)C]phosphatidylethanol was measured. The enhancing effect of TPA was almost completely blocked by a specific protein kinase C inhibitor, GF 109203X. In contrast, GF 109203X failed to inhibit the sphingosine, imipramine and propranolol stimulatory effects, indicating that their stimulation was independent of protein kinase C. The effect of TPA on phospholipase D was also blocked by imipramine and propranolol, whereas sphingosine additively potentiated TPA-mediated phospholipase D activity, both at shorter and longer (2-60 min) times of incubation. These results suggest that in glioma C6 cells, sphingosine is not only involved in a different phospholipase D activation than the TPA regulatory system, but also that it operates in a different compartment of the cell.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipase D
/
Propranolol
/
Esfingosina
/
Neoplasias Encefálicas
/
Acetato de Tetradecanoilforbol
/
Glioma
/
Imipramina
/
Isoenzimas
/
Proteínas de Neoplasias
Limite:
Animals
Idioma:
En
Revista:
Cell Signal
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Polônia