Sleep modifies glutamate decarboxylase mRNA within the barrel cortex of rats after a mystacial whisker trim.
Sleep
; 24(3): 261-6, 2001 May 01.
Article
em En
| MEDLINE
| ID: mdl-11322707
ABSTRACT
STUDY OBJECTIVES:
Determine the effects of time of day and sleep deprivation on glutamate decarboxylase 67,000 MW (GAD67) mRNA during cortical synaptic reorganization induced by a unilateral trimming of the mystacial vibrissae in rats.DESIGN:
Two experiments were conducted--One measured GAD67 mRNA in the barrel cortex at 3 or 6 h after a unilateral whisker cut at either light or dark onset; the other measured GAD67 mRNA after a unilateral whisker cut at light onset with or without sleep deprivation.SETTING:
University-based Research Laboratory.PARTICIPANTS:
Sprague-Dawley rats (250-350 g).INTERVENTIONS:
N/A. MEASUREMENTS ANDRESULTS:
The reverse transcriptase polymerase chain reaction was used to measure the time of day changes in GAD67 mRNA after a unilateral whisker cut. GAD67 mRNA decreased in the barrel cortex at 3 and 6 h after a whisker trim at dark onset when the rats were mainly awake. No changes were observed in GAD67 mRNA levels after a whisker cut at light onset, a time when the rats mainly sleep. In situ hybridization for GAD67 mRNA supported these findings; no changes in GAD mRNA occurred in layer 4 of the barrel cortex that received input from the mystacial whiskers that were trimmed at light onset. However, when the rats were sleep-deprived, GAD67 mRNA increased in the barrel cortex receiving input from the lip hairs surrounding the trimmed mystacial whiskers.CONCLUSIONS:
These data imply that sleep modifies GAD67 mRNA expression and that this effect is dependent upon the nature of the ongoing synaptic reorganization. They support the notion that sleep serves a synaptic function.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sono
/
Privação do Sono
/
Córtex Somatossensorial
/
RNA Mensageiro
/
Vibrissas
/
Glutamato Descarboxilase
Limite:
Animals
Idioma:
En
Revista:
Sleep
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Estados Unidos