Transcriptional repression of p21(waf1) promoter by hepatitis B virus X protein via a p53-independent pathway.
Gene
; 275(1): 163-8, 2001 Sep 05.
Article
em En
| MEDLINE
| ID: mdl-11574165
ABSTRACT
The X-gene product of hepatitis B virus (HBx) has been implicated in hepatitis B virus (HBV)-mediated hepatocellular carcinoma through its ability to induce liver cancer in some transgenic mice and to transactivate a variety of viral and cellular promoters. In this study, we demonstrated that the level of p21(waf1) RNA was decreased in the HBx-expressing cells and this effect was due to the transcriptional repression of the p21(waf1) gene by HBx via a p53-independent pathway. As the Sp1 binding sites of the p21(waf1) promoter were sufficient to confer HBx responsiveness to a previously non-responsive promoter, we suggested that HBx represses the transcription of p21(waf1) by downregulating the activity of Sp1. Because the tumor repressor p21(waf1) protein is a universal inhibitor of cyclin-CDK complexes and DNA replication that induces cell cycle arrest at the G1-S checkpoint, the repression of p21(waf1) by HBx might play an important role in a HBV-mediated pathogenesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transativadores
/
Proteína Supressora de Tumor p53
/
Regiões Promotoras Genéticas
/
Ciclinas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Gene
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Coréia do Sul