Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon-gamma and lipopolysaccharide.
Gen Pharmacol
; 35(1): 21-8, 2000 Jul.
Article
em En
| MEDLINE
| ID: mdl-11679202
ABSTRACT
It was found that the production of nitric oxide (NO) by RAW 264.7 macrophages stimulated with interferon-gamma (IFN-gamma) and lipopolysaccharide (LPS) could be markedly inhibited by the ethyl-acetate-soluble fraction of 80% aqueous methanolic extract of stem barks of Cudrania tricuspidata (EACT). Inhibition of NO production was achieved by reducing inducible nitric oxide synthase (iNOS) expression at protein and mRNA levels and by inactivating nuclear factor-kappa B (NF-kappa B), but not by inhibiting iNOS activity. Thus, further phytochemical and pharmacological studies may lead to isolation and structural identification of an inhibitor of iNOS from C. tricuspidata, which has been used as a traditional medicine for curing inflammation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lipopolissacarídeos
/
Interferon gama
/
Óxido Nítrico Sintase
/
Macrófagos
/
Acetatos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Gen Pharmacol
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Coréia do Sul