Human microglial cells express a functional IL-12 receptor and produce IL-12 following IL-12 stimulation.
Eur J Immunol
; 31(11): 3228-39, 2001 Nov.
Article
em En
| MEDLINE
| ID: mdl-11745339
ABSTRACT
Microglial cells (MC) are IL-12 producers in the central nervous system. Here, we found that IL-12 receptor subunits beta1 and beta2 were both constitutively expressed, and up-regulated by IFN-gamma, in human primary MC. IL-12p70, after binding to its receptor, is internalized into vesicles that qualify as early endosomes as indicated by intracellular colocalization with transferrin. IL-12 induced tyrosine phosphorylation and nuclear translocation of STAT4. IL-12 signaling in human MC also involved members of the NFkappaB family. IL-12p70 and, more effectively, the combination of IL-12p70 and IFN-gamma, induced IL-12p40 mRNA expression and bioactive IL-12p70 production. Human MC, thus, express a functional IL-12 receptor and produce bioactive IL-12 following IL-12 stimulation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Interleucina
/
Microglia
/
Interleucina-12
Limite:
Humans
Idioma:
En
Revista:
Eur J Immunol
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
França