Respiratory syncytial virus fusion protein mediates inhibition of mitogen-induced T-cell proliferation by contact.
J Virol
; 76(3): 1163-70, 2002 Feb.
Article
em En
| MEDLINE
| ID: mdl-11773392
ABSTRACT
Human respiratory syncytial virus (HRSV) and bovine respiratory syncytial virus (BRSV) are major pathogens in infants and calves, respectively. Experimental BRSV infection of calves and lambs is associated with lymphopenia and a reduction in responsiveness of peripheral blood lymphocytes (PBLs) to mitogens ex vivo. In this report, we show that in vitro mitogen-induced proliferation of PBLs is inhibited after contact with RSV-infected and UV-inactivated cells or with cells expressing RSV envelope proteins on the cell surface. The protein responsible was identified as the RSV fusion protein (F), as cells infected with a recombinant RSV expressing F as the single envelope protein or cells transfected with a plasmid encoding F were able to induce this effect. Thus, direct contact with RSV F is necessary and sufficient to inhibit proliferation of PBLs. Interestingly, F derived from HRSV was more efficient in inhibiting human PBL proliferation, while F from BRSV was more efficient in inhibiting bovine PBLs. Since various T-cell activation markers were upregulated after presenter cell contact, T lymphocytes are viable and may still be activated by mitogen. However, a significant fraction of PBLs were delayed or defective in G0/G1 to S-phase transit.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Linfócitos T
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Vírus Sincicial Respiratório Humano
/
Vírus Sincicial Respiratório Bovino
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Virol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Alemanha