Generation of adult-like antibody avidity profiles after early-life immunization with protein vaccines.
Eur J Immunol
; 32(3): 752-60, 2002 03.
Article
em En
| MEDLINE
| ID: mdl-11870619
ABSTRACT
The capacity to induce high-avidity antibodies following early-life immunization has long been questioned, and the possibility of inducing such antibodies soon after birth is a recognized goal for a number of vaccination strategies. Therefore, we assessed the capacity to develop high-avidity antibodies to peptidic vaccines in 1-week-old BALB/c mice. The dynamics of the generation of antibody molecules of increasing avidity were analyzed on circulating serum antibodies and, where feasible, at the single-cell level on spleen and bone marrow antibody-secreting cells. Two alum-adsorbed protein-based human vaccines, tetanus toxoid (TT) and pertussis toxin, induced neonatal antibody responses with adult-like avidity profiles. This was confirmed at the level of spleen and bone marrow ASC. In contrast, immunization with TT-P30, a 21-mer synthetic peptide containing a TT-immunodominant epitope, trinitrophenyl hapten (TNP) conjugated to ovalbumin or TNP conjugated to Ficoll, induced a much lower avidity profile in early life than in adults. These observations indicate that in murine models the avidity maturation of T cell-dependent antibody responses induced by structurally complex protein vaccines can be fully elicited after early life immunization, as opposed to the maturation of responses induced with short peptides or hapten-based vaccines.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunização
/
Vacinas de Subunidades Antigênicas
/
Ficoll
/
Sistema Imunitário
/
Anticorpos Antibacterianos
/
Afinidade de Anticorpos
Limite:
Animals
Idioma:
En
Revista:
Eur J Immunol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Suíça