Further evidence that a cell wall precursor [C(55)-MurNAc-(peptide)-GlcNAc] serves as an acceptor in a sorting reaction.
J Bacteriol
; 184(8): 2141-7, 2002 Apr.
Article
em En
| MEDLINE
| ID: mdl-11914345
ABSTRACT
Previous studies suggested that a Gly-containing branch of cell wall precursor [C(55)-MurNAc-(peptide)-GlcNAc], which is often referred to as lipid II, might serve as a nucleophilic acceptor in sortase-catalyzed anchoring of surface proteins in Staphylococcus aureus. To test this hypothesis, we first simplified the procedure for in vitro biosynthesis of Gly-containing lipid II by using branched UDP-MurNAc-hexapeptide isolated from the cytoplasm of Streptomyces spp. Second, we designed a thin-layer chromatography-based assay in which the mobility of branched but not linear lipid II is shifted in the presence of both sortase and LPSTG-containing peptide. These results and those of additional experiments presented in this study further suggest that lipid II indeed serves as a natural substrate in a sorting reaction.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Streptomyces
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Uridina Difosfato Ácido N-Acetilmurâmico
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Peptidoglicano
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Parede Celular
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Aminoaciltransferases
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos