p21-activated protein kinase gamma-PAK in pituitary secretory granules phosphorylates prolactin.
FEBS Lett
; 515(1-3): 84-8, 2002 Mar 27.
Article
em En
| MEDLINE
| ID: mdl-11943200
ABSTRACT
p21-activated protein kinase gamma-PAK phosphorylates prolactin (PRL) in rat pituitary secretory granules on Ser-177 and on the equivalent site, Ser-179, in recombinant human PRL. This is shown by comparison of phosphopeptide maps with the human PRL mutant S179D. gamma-PAK is present in rat and bovine granules as identified by in-gel phosphorylation of histone H4, and by immunoblotting. Thus, phosphorylation of PRL by gamma-PAK in granules produces the PRL molecule that has been shown to antagonize the growth-promoting activity of unmodified PRL, and is consistent with the identified role of gamma-PAK in the induction and maintenance of cytostasis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hipófise
/
Prolactina
/
Proteínas Serina-Treonina Quinases
/
Vesículas Secretórias
Limite:
Animals
/
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos