The pig platelet-activating factor receptor gene is expressed at the mRNA level in different tissues and is mapped to chromosome 6.
Anim Reprod Sci
; 70(3-4): 277-82, 2002 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-11943497
ABSTRACT
After the pig platelet-activating factor receptor (PAFr) gene was cloned and sequenced, the chromosomal location of this gene was studied using a pig/rodent somatic cell hybrid panel containing 27 cell lines. The results indicated that the pig PAFr gene is located on SSC6q22-23. Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is thought to be very important in the animal reproductive processes. Its function is mediated through a membrane-bound receptor. Pig PAFr mRNA distribution in different tissues was tested using reverse transcription and PCR (RT-PCR) reactions. All tissues examined expressed PAFr. Using a pig PAFr gene DNA competitor, PAFr expression was quantificated. The pig PAFr mRNA expression level was estimated to be from 1 x 10(2) to 1.2 x 10(4) copies of complementary DNA (cDNA) per 50 ng of total RNA. The highest level was found in lung, and the lowest in the skeletal muscle. These results demonstrated that PAFr was differentially expressed in pig tissues.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Suínos
/
RNA Mensageiro
/
Glicoproteínas da Membrana de Plaquetas
/
Expressão Gênica
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Mapeamento Cromossômico
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Receptores de Superfície Celular
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Receptores Acoplados a Proteínas G
Limite:
Animals
Idioma:
En
Revista:
Anim Reprod Sci
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos