The Role of DNA Polymerase delta in HeLa Cell DNA Replication Studied by Antisense Technology.

ABSTRACT

We have investigated the biological role of DNA polymerase delta in HeLa cell DNA replication using antisense technology with the Lipofectin Delivery and the GPT selection method. Both of the oligonucleotides designed to inhibit the expression of DNA polymerase delta and alpha can specifically reduce the DNA replication level in HeLa cells. This is the first report directly proving that DNA polymerase delta plays an important role in mammalian cell DNA replication.