Identification of up-regulated genes in human uterine leiomyoma by suppression subtractive hybridization.
Cell Res
; 12(3-4): 215-21, 2002 Sep.
Article
em En
| MEDLINE
| ID: mdl-12296380
ABSTRACT
In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression subtractive hybridization was used to construct an UL up-regulated library, which turned out to represent 88 genes. After two rounds of screening by reverse Northern analysis, twenty genes were proved to be up-regulated, including seventeen known genes and three genes with unknown function. All these genes were firstly associated with UL. Three genes with notable difference were selected for Northern confirmation. Our results proved the authenticity of the twenty genes. One gene named Phospholipase A2 (PLA2) showed up-regulation in 4/6 of the patients and investigation of tissue distribution indicated that it had obvious expression in prostate, testis, liver, heart and skeletal muscle.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Uterinas
/
Regulação para Cima
/
Leiomioma
/
Hibridização de Ácido Nucleico
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
/
Female
/
Humans
Idioma:
En
Revista:
Cell Res
Ano de publicação:
2002
Tipo de documento:
Article