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Expression of mRNA transcripts for ATP-sensitive potassium channels in human myometrium.
Curley, M; Cairns, M T; Friel, A M; McMeel, O M; Morrison, J J; Smith, T J.
Afiliação
  • Curley M; National Diagnostics Centre, National University of Ireland, Galway, Ireland.
Mol Hum Reprod ; 8(10): 941-5, 2002 Oct.
Article em En | MEDLINE | ID: mdl-12356945
The molecular mechanisms regulating human uterine quiescence and parturition are poorly understood. Potassium channels are central to regulation of cell membrane potential and contractility of smooth muscle. The aim of this study was to examine the expression of ATP-sensitive potassium channel (K(ATP) channel) subunits in human myometrium, and to investigate for possible differential expression of these subunits in myometrium obtained from three different functional states: (i) non-pregnant (NP); (ii) late pregnant not in labour (PNL); and (iii) late pregnant in labour (PL). RT-PCR detected the presence of mRNA for four subunits of K(ATP) channels (Kir6.1, Kir6.2, SUR1 and SUR2B) in the three tissue types. Quantitative analysis of these subunits was achieved with real-time RT-PCR using Lightcycler(TM) technology. This analysis showed that there were significantly higher levels of Kir6.1 and SUR2B transcripts in NP myometrium compared with those measured in myometrium obtained during pregnancy (P < 0.001). Lower levels of Kir6.2 and SUR1 mRNA expression were found, although higher transcript levels in NP myometrium (P < 0.05) were still observed. Our results indicate that the major K(ATP) channel expressed in human myometrium is composed of Kir6.1 and SUR2B, and that down-regulation of this channel may facilitate myometrial function during late pregnancy.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Canais de Potássio / Trifosfato de Adenosina / Transportadores de Cassetes de Ligação de ATP / Miométrio Tipo de estudo: Diagnostic_studies Limite: Adult / Female / Humans / Pregnancy Idioma: En Revista: Mol Hum Reprod Assunto da revista: BIOLOGIA MOLECULAR / MEDICINA REPRODUTIVA Ano de publicação: 2002 Tipo de documento: Article País de afiliação: Irlanda
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Canais de Potássio / Trifosfato de Adenosina / Transportadores de Cassetes de Ligação de ATP / Miométrio Tipo de estudo: Diagnostic_studies Limite: Adult / Female / Humans / Pregnancy Idioma: En Revista: Mol Hum Reprod Assunto da revista: BIOLOGIA MOLECULAR / MEDICINA REPRODUTIVA Ano de publicação: 2002 Tipo de documento: Article País de afiliação: Irlanda