Factors affecting the composition of oligosaccharides produced in chitosan hydrolysis using immobilized chitosanases.
Biotechnol Prog
; 18(5): 969-74, 2002.
Article
em En
| MEDLINE
| ID: mdl-12363347
ABSTRACT
The hydrolysis reaction of chitosan using immobilized chitosanases with regard to the composition of its products and the yield of the intermediate target products, pentamer and hexamer of chitosan oligosaccharides, was investigated. Chitosanase was immobilized onto agar or agarose gel particles by the multipoint attachment method. In batch experiments, surface enzyme density, support particle size, temperature, agitator speed, and initial substrate concentration significantly affected the composition of the oligosaccharides produced. It was believed that these factors all related to the reaction rate and mass transfer rate at the surface of the support materials immobilizing the enzymes. These effects were summarized as a correlation with Damköhler number (Da), defined as the ratio of the maximum reaction rate to the maximum mass transfer rate. The result showed that the reaction conditions that give a low value of Da provide a high yield of pentamer and hexamer oligosaccharides.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
/
Sefarose
/
Quitina
/
Ágar
/
Enzimas Imobilizadas
/
Glicosídeo Hidrolases
Idioma:
En
Revista:
Biotechnol Prog
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Japão