An improved method for acetaldehyde determination in blood by high-performance liquid chromatography and solid-phase extraction.
J Chromatogr Sci
; 40(9): 477-82, 2002 Oct.
Article
em En
| MEDLINE
| ID: mdl-12433107
ABSTRACT
This study reports on an improved method for acetaldehyde (ACH) determination in blood by high-performance liquid chromatography (HPLC). In the case of HPLC analysis, ACH is generally converted to derivatives for ultraviolet detection (for example 2,4-dinitrophenylhydrazine [DNPH] derivative). Nevertheless, elevation of the background during protein precipitation, hydrazone synthesis, or both frequently results in a serious loss of accuracy and precision of the analysis. The method in this study is developed to minimize the increase in nonspecific ACH-DNPH with a view to optimize mainly the synthetic condition of ACH-DNPH. The background is decreased dramatically by gentle deproteination, optimization of the DNPH amount and reaction pH, and reversed-phase solid extraction for the elimination of excess DNPH reagent. The standard curves show good linearity between 0 and 100 microM and minimal background is observed, indicating that the method is useful for monitoring the ACH concentration in blood.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Acetaldeído
Limite:
Humans
Idioma:
En
Revista:
J Chromatogr Sci
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Japão