HPLC assay for methylmalonyl-CoA epimerase.
Anal Bioanal Chem
; 375(3): 344-9, 2003 Feb.
Article
em En
| MEDLINE
| ID: mdl-12589497
ABSTRACT
Methylmalonyl-CoA epimerase (MCE) is broadly distributed in nature and has diverse cellular roles. Many MCE homologues are represented in public databases, but the biochemical function and physiological roles of the majority of these putative proteins have not been investigated. Here, a simplified assay for MCE is described. In this assay, MCE converted (2S)-methylmalonyl-CoA to (2R)-methylmalonyl-CoA which in turn was converted to succinyl-CoA by methylmalonyl-CoA mutase, an enzyme specific for the 2 R isomer. MCE activity was quantified by measuring the disappearance of methylmalonyl-CoA by HPLC. To obtain the methylmalonyl-CoA mutase which was required as a reagent for the assay, an Escherichia coli strain was constructed that expressed high levels of this enzyme as a fusion protein with an 8x histidine tag. This allowed purification of the mutase in a single affinity chromatography step. Previously reported MCE assays required radioactive substrates and/or multiple reagent enzymes that were difficult to obtain. The assay reported here overcomes these difficulties and hence will facilitate studies of MCEs. Such enzymes play important roles in the metabolism of both prokaryotes and higher eukaryotes including humans.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Racemases e Epimerases
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Bioanal Chem
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Estados Unidos