Saturation of two chromosome regions conferring resistance to SCMV with SSR and AFLP markers by targeted BSA.
Theor Appl Genet
; 106(3): 485-93, 2003 Feb.
Article
em En
| MEDLINE
| ID: mdl-12589549
ABSTRACT
Quantitative trait loci (QTLs) and bulked segregant analyses (BSA) identified the major genes Scmv1 on chromosome 6 and Scmv2 on chromosome 3, conferring resistance against sugarcane mosaic virus (SCMV) in maize. Both chromosome regions were further enriched for SSR and AFLP markers by targeted bulked segregant analysis (tBSA) in order to identify and map only markers closely linked to either Scmv1 or Scmv2. For identification of markers closely linked to the target genes, symptomless individuals of advanced backcross generations BC5 to BC9 were employed. All AFLP markers, identified by tBSA using 400 EcoRI/ MseI primer combinations, mapped within both targeted marker intervals. Fourteen SSR and six AFLP markers mapped to the Scmv1 region. Eleven SSR and 18 AFLP markers were located in the Scmv2 region. Whereas the linear order of SSR markers and the window size for the Scmv2 region fitted well with publicly available genetic maps, map distances and window size differed substantially for the Scmv1 region on chromosome 6. A possible explanation for the observed discrepancies is the presence of two closely linked resistance genes in the Scmv1 region.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes de Plantas
/
Folhas de Planta
/
Zea mays
/
Imunidade Inata
/
Vírus do Mosaico
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Theor Appl Genet
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Alemanha