Expression, purification and preliminary crystallographic analysis of human sorbitol dehydrogenase.
Acta Crystallogr D Biol Crystallogr
; 59(Pt 3): 558-60, 2003 Mar.
Article
em En
| MEDLINE
| ID: mdl-12595725
ABSTRACT
Human sorbitol dehydrogenase (SDH) was expressed in Escherichia coli BL21 cells and purified using ammonium sulfate precipitation and anion-exchange and dye-affinity chromatography. Purified SDH was crystallized from polyethylene glycol solutions using the hanging-drop vapour-diffusion method. X-ray data were collected to 2.75 A resolution. The crystals belong to the monoclinic C2 space group, with unit-cell parameters a = 145.9, b = 52.3, c = 169.0 A, beta = 101.8 degrees. This is the first crystallization report of human sorbitol dehydrogenase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
L-Iditol 2-Desidrogenase
Limite:
Humans
Idioma:
En
Revista:
Acta Crystallogr D Biol Crystallogr
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Austrália