Gene expression markers for Caenorhabditis elegans vulval cells.
Gene Expr Patterns
; 2(3-4): 235-41, 2002 Dec.
Article
em En
| MEDLINE
| ID: mdl-12617807
ABSTRACT
The analysis of cell fate patterning during the vulval development of Caenorhabditis elegans has relied mostly on the direct observation of cell divisions and cell movements (cell lineage analysis). However, reconstruction of the developing vulva from EM serial sections has suggested seven different cell types (vulA, vulB1, vulB2, vulC, vulD, vulE, and vulF), many of which cannot be distinguished based on such observations. Here we report the vulval expression of seven genes, egl-17, cdh-3, ceh-2, zmp-1, B0034.1, T04B2.6 and F47B8.6 based on gfp, cfp and yfp (green fluorescent protein and color variants) reporter fusions. Each gene expresses in a specific subset of vulval cells, and is therefore useful as a marker for vulval cell fates. Together, expressions of markers distinguish six cell types, and reveal a strict temporal control of gene expression in the developing vulva.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vulva
/
Caenorhabditis elegans
/
Perfilação da Expressão Gênica
Limite:
Animals
Idioma:
En
Revista:
Gene Expr Patterns
Assunto da revista:
BIOLOGIA MOLECULAR
/
CEREBRO
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos