Ex vivo expansion of human adult stem cells capable of primary and secondary hemopoietic reconstitution.
Exp Hematol
; 31(3): 261-70, 2003 Mar.
Article
em En
| MEDLINE
| ID: mdl-12644024
ABSTRACT
OBJECTIVE:
Ex vivo expansion of human hemopoietic stem cells (HSC) is an important issue in transplantation and gene therapy. Encouraging results have been obtained with cord blood, where extensive amplification of primitive progenitors was observed. So far, this goal has been elusive with adult cells, in which amplification of committed and mature cells, but not of long-term repopulating cells, has been described.METHODS:
Adult normal bone marrow (BM) and mobilized peripheral blood (MPB) CD34(+) cells were cultured in a stroma-free liquid culture in the presence of Flt-3 ligand (FL), thrombopoietin (TPO), stem cell factor (SCF), interleukin-6 (IL-6), or interleukin-3 (IL-3). Suitable aliquots of cells were used to monitor cell production, clonogenic activity, LTC-IC output, and in vivo repopulating capacity.RESULTS:
Here we report that BM and MPB HSC can be cultured in the presence of FL, TPO, SCF, and IL-6 for up to 10 weeks, during which time they proliferate and produce large numbers of committed progenitors (up to 3000-fold). Primitive NOD/SCID mouse repopulating stem cells (SRC) are expanded sixfold after 3 weeks (by limiting dilution studies) and retain the ability to repopulate secondary NOD/SCID mice after serial transplants. Substitution of IL-6 with IL-3 leads to a similarly high production of committed and differentiated cells but only to a transient (1 week) expansion of SRC(s), which do not possess secondary repopulation capacity.CONCLUSION:
We report evidence to show that under appropriate culture conditions, adult human SRC can also be induced to expand with limited differentiation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Hematopoéticas
/
Transplante de Células-Tronco Hematopoéticas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Exp Hematol
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Itália