Reconstruction of epidermis by grafting of keratinocytes cultured on polymer support--clinical study.
Int J Dermatol
; 42(3): 219-23, 2003 Mar.
Article
em En
| MEDLINE
| ID: mdl-12653921
ABSTRACT
BACKGROUND:
Extensive wound coverage still represents a challenge for contemporary medicine. We demonstrate the results of a clinical trial of the grafting of cultured keratinocytes directly on a polymer cultivation support in the treatment of skin defects in seriously burned patients and in patients with trophic ulcers.METHODS:
Wound closure was evaluated clinically. The morphology and phenotypic pattern of the reconstructed epidermis, including the basal lamina, as well as the presence of Langerhans cells, were evaluated immunocytochemically using a panel of monoclonal antibodies.RESULTS:
All layers of the reconstructed epidermis were normally differentiated (cytokeratin immunocytochemistry). The basal lamina contained collagen type IV and laminin. The reconstructed epidermis was extensively colonized by Langerhans cells.CONCLUSIONS:
The results of the described technology are encouraging, especially in patients after a burn injury. The described procedure is suitable for the treatment of skin defects in clinical practice.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Queratinócitos
/
Transplante de Células
/
Engenharia Tecidual
/
Epiderme
Limite:
Adult
/
Aged
/
Aged80
/
Child
/
Female
/
Humans
/
Infant
/
Male
/
Middle aged
Idioma:
En
Revista:
Int J Dermatol
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
República Tcheca