Efficient therapeutic gene delivery after systemic administration of a novel polyethylenimine/DNA vector in an orthotopic bladder cancer model.
Cancer Res
; 63(14): 4017-20, 2003 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-12874000
ABSTRACT
Successful systemic gene therapy has been hindered by vector-related limitations, including toxicity and inefficient gene delivery to tumor cells after i.v. administration. To circumvent these problems, we developed a novel formulation between the polycation polyethyleneimine and DNA that mediates high-level tumor cell transduction in vitro and efficient i.v. gene delivery in that greater reporter gene expression occurred in tumor than in lung. Strikingly, administration of just 6 micro g of the polyethyleneimine/DNA-p53 vector every 3 days for 3 weeks indicated restoration of normal cell cycle regulation and apoptotic mechanisms as demonstrated by efficient p53 expression, increased apoptosis, and a 70% reduction in tumor size in an orthotopic bladder cancer model. This novel vector formulation represents a new method to increase i.v. delivery of genes to tumors.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polietilenoimina
/
Neoplasias da Bexiga Urinária
/
DNA
/
Terapia Genética
/
Vetores Genéticos
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Estados Unidos