[Production and application of rabbit anti-imitative spider dragline silk protein polyclonal antibody].
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
; 35(8): 756-60, 2003 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-12897973
ABSTRACT
A synthetic spider dragline gene s600 was cloned into fusion protein expression vector pGEX-KG and expressed in Escherichia coli. Protein S600 was purified and rabbit antiserum was prepared. Amino acid composition analysis confirmed the right expression of S600. Western blot analysis revealed that anti-S600 antiserum could react with natural spider silk, so the synthetic dragline protein, designed by the authors, shares similar immunological characteristics with the natural spider silk. An ELISA system was also established for the quantitative detection of synthetic dragline protein expression in silk gland (or in the cocoon) of transgenic silkworm.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aranhas
/
Proteínas Recombinantes de Fusão
/
Proteínas
/
Fibroínas
/
Soros Imunes
/
Anticorpos
Limite:
Animals
Idioma:
Zh
Revista:
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
China