Cloning and characterization of the Dictyostelium discoideum rasG genomic sequences.
Biochim Biophys Acta
; 1130(1): 85-9, 1992 Feb 28.
Article
em En
| MEDLINE
| ID: mdl-1339294
ABSTRACT
A Dictyostelium discoideum genomic DNA clone containing the ras-related gene, rasG was isolated using the rasG cDNA as a probe. The genomic clone encompasses the entire coding region of the gene and 1.5 kb of 5' flanking region. The rasG gene contains a single intron as determined by sequence comparison with the cDNA, whereas the highly related rasD gene contains three introns. Primer extension analysis showed that transcription of the rasG gene initiates at multiple sites. Sequence analysis of the 5' flanking region of the gene revealed a stretch of thymine residues upstream from the transcription start sites but there is no evidence for a TATA box sequence.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes ras
/
Dictyostelium
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Canadá