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Cloning and characterization of the Dictyostelium discoideum rasG genomic sequences.
Robbins, S M; Williams, J G; Spiegelman, G B; Weeks, G.
Afiliação
  • Robbins SM; Department of Microbiology, University of British Columbia, Vancouver, Canada.
Biochim Biophys Acta ; 1130(1): 85-9, 1992 Feb 28.
Article em En | MEDLINE | ID: mdl-1339294
ABSTRACT
A Dictyostelium discoideum genomic DNA clone containing the ras-related gene, rasG was isolated using the rasG cDNA as a probe. The genomic clone encompasses the entire coding region of the gene and 1.5 kb of 5' flanking region. The rasG gene contains a single intron as determined by sequence comparison with the cDNA, whereas the highly related rasD gene contains three introns. Primer extension analysis showed that transcription of the rasG gene initiates at multiple sites. Sequence analysis of the 5' flanking region of the gene revealed a stretch of thymine residues upstream from the transcription start sites but there is no evidence for a TATA box sequence.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Genes ras / Dictyostelium Limite: Animals Idioma: En Revista: Biochim Biophys Acta Ano de publicação: 1992 Tipo de documento: Article País de afiliação: Canadá
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Genes ras / Dictyostelium Limite: Animals Idioma: En Revista: Biochim Biophys Acta Ano de publicação: 1992 Tipo de documento: Article País de afiliação: Canadá