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Conformational integrity of a recombinant toxoid of Pseudomonas aeruginosa exotoxin A containing a deletion of glutamic acid-553.
Killeen, K P; Collier, R J.
Afiliação
  • Killeen KP; Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Biochim Biophys Acta ; 1138(2): 162-6, 1992 Feb 14.
Article em En | MEDLINE | ID: mdl-1347236
A mutant form of Pseudomonas aeruginosa exotoxin A (ETA) carrying a deletion of glutamic acid-553, an important active-site residue, was expressed in an ETA-negative strain of P. aeruginosa and shown to be exported from the cells as efficiently as wild-type ETA. The mutant protein, purified from the culture medium, was devoid of ADP-ribosyltransferase activity. Protein conformation was barely perturbed by the deletion, as determined by a number of measures, including affinity for substrate NAD, proteinase sensitivity, absorbance and fluorescence spectroscopy, and differential scanning calorimetry. The conformational integrity and stability of the mutant toxin are consistent with potential use of the protein in vaccines or as a carrier in preparing conjugate vaccines.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Toxinas Bacterianas / Toxoides / ADP Ribose Transferases / Fatores de Virulência / Exotoxinas / Glutamatos Idioma: En Revista: Biochim Biophys Acta Ano de publicação: 1992 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Toxinas Bacterianas / Toxoides / ADP Ribose Transferases / Fatores de Virulência / Exotoxinas / Glutamatos Idioma: En Revista: Biochim Biophys Acta Ano de publicação: 1992 Tipo de documento: Article