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Association between promyelocyte protein and small ubiquitin-like modifier protein and the progression of cervical neoplasia.
Szendefi, Melinda; Walt, Heinrich; Krasieva, Tatiana B; Caduff, Rosmarie; Osann, Kathryn E; LaMorte, Vickie J.
Afiliação
  • Szendefi M; Department of Obstetrics and Gynecology, University Hospital, Zurich, Switzerland.
Obstet Gynecol ; 102(6): 1269-77, 2003 Dec.
Article em En | MEDLINE | ID: mdl-14662214
OBJECTIVE: To examine the association between the size and number of promyelocyte protein-containing nuclear bodies, their colocalization with the small ubiquitin-like modifier protein, and existing histopathologic staging of cervical neoplasia progressing toward squamous cell carcinoma. METHODS: Fluorescence-based immunodetection of the promyelocyte protein and the small ubiquitin-like modifier protein was performed on paraffin-embedded and histopathologically graded human uterine cervical tissues. Quantitative measurements of the size and number of the promyelocyte protein-containing nuclear bodies were made and statistically analyzed. RESULTS: We found that promyelocyte protein-containing nuclear bodies exhibit changes in both size and number throughout the continuum of cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma. An increase in number and size of the bodies occurs with progression from normal to CIN I/CIN II. In CIN III, two new subcategories of nuclear body are present with distinctly different promyelocyte protein patterns, with the type B CIN III losing the small ubiquitin-like modifier protein partnership. In squamous cell carcinoma, we see the loss of this colocalization in both well and poorly differentiated tumors, with a distinctly different promyelocyte protein pattern. Well-differentiated tumors have bigger nuclear bodies that are more numerous than those of the poorly differentiated tumors. CONCLUSION: These data support the use of promyelocyte and small ubiquitin-like modifier proteins as a cytodiagnostic marker that parallels cervical cancer progression.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Proteínas Nucleares / Carcinoma de Células Escamosas / Ubiquitinas / Displasia do Colo do Útero / Neoplasias do Colo do Útero / Proteínas de Neoplasias Tipo de estudo: Risk_factors_studies Limite: Adult / Aged / Aged80 / Female / Humans / Middle aged Idioma: En Revista: Obstet Gynecol Ano de publicação: 2003 Tipo de documento: Article País de afiliação: Suíça
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Proteínas Nucleares / Carcinoma de Células Escamosas / Ubiquitinas / Displasia do Colo do Útero / Neoplasias do Colo do Útero / Proteínas de Neoplasias Tipo de estudo: Risk_factors_studies Limite: Adult / Aged / Aged80 / Female / Humans / Middle aged Idioma: En Revista: Obstet Gynecol Ano de publicação: 2003 Tipo de documento: Article País de afiliação: Suíça