Hyperoxia-induced DNA damage causes decreased DNA methylation in human lung epithelial-like A549 cells.
Antioxid Redox Signal
; 6(1): 129-36, 2004 Feb.
Article
em En
| MEDLINE
| ID: mdl-14713344
ABSTRACT
The effect of hyperoxia on levels of DNA damage and global DNA methylation was examined in lung epithelial-like A549 cells. DNA damage was assessed by the single-cell gel electrophoresis (comet assay) and DNA methylation status by the cytosine extension assays. Cells exposed to ionizing radiation (0, 1, 2, 4, or 8 Gy) showed increasing rates of percentage of DNA in the tail and tail length with increasing radiation dose. When cells were exposed to room air (normoxia) for 1 day and 95% O2 (hyperoxia) for 1, 2, 3, 4, and 5 days, data indicated that hyperoxia caused time-dependent increases in levels of (a) single strand breaks, (b) double strand breaks, and (c) 8-oxoguanine. Decreased DNA methylation also was observed at day 5 of hyperoxic exposure, suggesting that hyperoxia-induced DNA damage can influence patterns of DNA methylation in a lung-derived cell line.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxigênio
/
Dano ao DNA
/
Hiperóxia
/
Metilação de DNA
/
Células Epiteliais
/
Guanina
Tipo de estudo:
Etiology_studies
Limite:
Humans
Idioma:
En
Revista:
Antioxid Redox Signal
Assunto da revista:
METABOLISMO
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos