LRH-1/hB1F and HNF1 synergistically up-regulate hepatitis B virus gene transcription and DNA replication.
Cell Res
; 13(6): 451-8, 2003 Dec.
Article
em En
| MEDLINE
| ID: mdl-14728801
ABSTRACT
Enhancer II (ENII) is one of the critical cis-elements in the Hepatitis B Virus (HBV) genome for the hepatic viral gene transcription and DNA replication. The liver-specific activity of ENII is regulated by multiple liver-enriched transcription factors, including LRH-1/hB1F, HNF1, HNF3b, HNF4 and C/EBP. Knowledge on the interplay of these important factors is still limited. In this study, we demonstrate a functional synergism between the orphan nuclear receptor LRH-1/hB1F and the homeoprotein HNF1 in up-regulating the liver-specific activity of ENII. This synergism is sufficient for initiating the viral gene transcription and DNA replication in non-hepatic cells. We have defined the activation domains in hB1F and HNF1 that contribute to the synergism. We further show that hB1F and HNF1 can interact directly in vitro and have mapped the domains required for this interaction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Replicação Viral
/
Proteínas Nucleares
/
Regulação Viral da Expressão Gênica
/
Transativadores
/
Vírus da Hepatite B
/
Proteínas de Ligação a DNA
/
Replicação do DNA
Limite:
Humans
Idioma:
En
Revista:
Cell Res
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
China