Improved survival of orthotopic liver allograft in swine by addition of trophic factors to University of Wisconsin solution.
Transplantation
; 77(2): 302-19, 2004 Jan 27.
Article
em En
| MEDLINE
| ID: mdl-14742997
ABSTRACT
Serum-free preservation media such as University of Wisconsin (UW) may cause tissue damage through trophic factor (TF) deprivation. This study evaluated whether the addition of TFs to UW solution improves liver graft quality after extended cold preservation time in pigs. UW solution was supplemented with epidermal growth factor, insulin-like growth factor-1, nerve growth factor-beta, bactenecin, and substance P to create TF-supplemented (TFS) UW. Orthotopic liver transplantation was performed after 18 hr of static cold storage at 4 degrees C in UW (n=7) or TFS-UW (n=7) solution. Recipients of grafts preserved with TFS-UW demonstrated significantly better 5-day survival (57%) than those preserved with UW alone (14%) (P<0.05). Adenosine triphosphate content in grafts preserved in TFS-UW was significantly higher than in grafts preserved in UW (17.4+/-5.0 vs. 4.8+/-1.2 nmol/mg protein, respectively) (P<0.05). This study showed that the addition of TFs to UW solution allowed a significant extension of cold ischemic time in pigs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rafinose
/
Adenosina
/
Alopurinol
/
Transplante de Fígado
/
Substâncias de Crescimento
/
Glutationa
/
Sobrevivência de Enxerto
/
Insulina
/
Fígado
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Transplantation
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Bélgica