Evaluation of a monolithic epoxy silica support for penicillin G acylase immobilization.
J Chromatogr A
; 1031(1-2): 93-100, 2004 Mar 26.
Article
em En
| MEDLINE
| ID: mdl-15058571
ABSTRACT
In this work, a new type of penicillin G acylase (PGA)-based monolithic silica support was developed and evaluated for the chiral separation in HPLC. The preparation procedure consisted of two steps:
preparation of an epoxy derivatized monolithic silica column and chemical modification of the epoxide groups with the enzyme chiral selector. The epoxy Silica-Rod column for the immobilization of PGA was prepared with the in situ modification process by using epoxy-silanes and the identification of the species bound to the surface was achieved by solid-state nuclear magnetic resonance. The enzyme was covalently immobilized to the surface of the derivatized monolithic column. The enantioselectivity and the performance of the developed column are discussed and compared to the corresponding experimental data obtained with a PGA-based microparticulate (5 microm) silica column.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Penicilina Amidase
/
Dióxido de Silício
/
Enzimas Imobilizadas
/
Compostos de Epóxi
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Itália