[Bioinformatics analysis to the differentially expressed genes of normal mucosa and carcinoma of colon].
Zhejiang Da Xue Xue Bao Yi Xue Ban
; 33(2): 95-101, 124, 2004 03.
Article
em Zh
| MEDLINE
| ID: mdl-15067726
ABSTRACT
OBJECTIVE:
To systemically analyze the differentially expressed genes from normal mucosa and carcinoma of colon obtained by SSH method.METHODS:
An automatic platform for the analysis of nucleotides based on Linux was constructed, and one of the three subtracted libraries from SSH (T -N) was systematically analyzed by this platform. Part of the results was verified by semi- quantity RT-PCR.RESULT:
The automatic platform for the analysis of nucleotides based on Linux was successfully constructed. There were 15 contigs from the subtracted T-N libraries, among which 2 had no match with known genes in the GenBank. The expressions of genes Homo Sapiens thymosin beta 4 (THY) and Homo Sapiens HbxAg transactivated protein 2 (XTP) had trends of increase with the progress of normal tissue-adenoma-adenocarcinoma when verified by semi- quantity RT-PCR.CONCLUSION:
Linux-based automatic platform provides an efficient way to systematically analyze the nucleotide sequences, which may be used in study on the mechanism of colorectal carcinogenesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Colo
/
Neoplasias do Colo
/
Biologia Computacional
/
Perfilação da Expressão Gênica
/
Mucosa Intestinal
Limite:
Female
/
Humans
/
Male
Idioma:
Zh
Revista:
Zhejiang Da Xue Xue Bao Yi Xue Ban
Assunto da revista:
MEDICINA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
China