High throughput screening technologies for direct cyclic AMP measurement.
Assay Drug Dev Technol
; 1(2): 291-303, 2003 Apr.
Article
em En
| MEDLINE
| ID: mdl-15090194
ABSTRACT
A study comparing five different cAMP detection technologies in terms of sensitivity, robustness, and feasibility for HTS is presented. In this report, the following methods are described a nonhomogeneous DELFIA, and the homogeneous methods based on time-resolved fluorescence (HTRF), luminescent singlet oxygen channeling or ALPHAScreen, FP, and high-affinity enzyme complementation. DELFIA had the highest sensitivity, whereas ALPHAScreen and HTRF shared several advantages, including high sensitivity, broad dynamic range, and minimal reagent addition steps. For G(s)-coupled antagonist screens, we found HTRF and ALPHAScreen the more sensitive and HTS-compatible techniques.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bioensaio
/
AMP Cíclico
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Revista:
Assay Drug Dev Technol
Assunto da revista:
FARMACOLOGIA
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Suíça